A versatile microfluidic device for multiple ex vivo/in vitro tissue assays unrestrained from tissue topography.

ABSTRACT: Precision-cut tissue slices are an important in vitro system to study organ function because they preserve most of the native cellular microenvironments of organs, including complex intercellular connections. However, during sample manipulation or slicing, some of the natural surface topology and structure of these tissues is lost or damaged. Here, we introduce a microfluidic platform to perform multiple assays on the surface of a tissue section, unhindered by surface topography. The device consists of a valve on one side and eight open microchannels located on the opposite side, with the tissue section sandwiched between these two structures. When the valve is actuated, eight independent microfluidic channels are formed over a tissue section. This strategy prevents cross-contamination whe

SUBMITTER: de Hoyos-Vega JM 

PROVIDER: S-EPMC8433291 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature