Monolithic integration of nanorod arrays on microfluidic chips for fast and sensitive one-step immunoassays.

ABSTRACT: Here, we present integrated nanorod arrays on microfluidic chips for fast and sensitive flow-through immunoassays of physiologically relevant macromolecules. Dense arrays of Au nanorods are easily fabricated through one-step oblique angle deposition, which eliminates the requirement of advanced lithography methods. We report the utility of this plasmonic structure to improve the detection limit of the cardiac troponin I (cTnI) assay by over 6 × 10⁵-fold, reaching down to 33.9 fg mL^-1 (~1.4 fM), compared with an identical assay on glass substrates. Through monolithic integration with microfluidic elements, the device enables a flow-through assay for quantitative detection of cTnI in the serum with a detection sensitivity of 6.9 pg mL^-1 (~0.3 pM) in <6 min, w

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8433357 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature