An aptamer-based shear horizontal surface acoustic wave biosensor with a CVD-grown single-layered graphene film for high-sensitivity detection of a label-free endotoxin.

ABSTRACT: The thickness of the sensitive layer has an important influence on the sensitivity of a shear horizontal surface acoustic wave (SH-SAW) biosensor with a delay-line structure and lower layer numbers of graphene produce better sensitivity for biological detection. Therefore, a label-free and highly sensitive SH-SAW biosensor with chemical vapor deposition (CVD-)-grown single-layered graphene (SLG) for endotoxin detection was developed in this study. With this methodology, SH-SAW biosensors were fabricated on a 36° Y-90° X quartz substrate with a base frequency of 246.2 MHz, and an effective detection cell was fabricated using acrylic material. To increase the surface hydrophilicity, chitosan was applied to the surface of the SLG film. Additionally, the aptamer was immobilized on the surface

SUBMITTER: Ji J 

PROVIDER: S-EPMC8433395 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature