A microfluidic platform for cultivating ovarian cancer spheroids and testing their responses to chemotherapies.

ABSTRACT: There is increasing interest in utilizing in vitro cultures as patient avatars to develop personalized treatment for cancer. Typical cultures utilize Matrigel-coated plates and media to promote the proliferation of cancer cells as spheroids or tumor explants. However, standard culture conditions operate in large volumes and require a high concentration of cancer cells to initiate this process. Other limitations include variability in the ability to successfully establish a stable line and inconsistency in the dimensions of these microcancers for in vivo drug response measurements. This paper explored the utility of microfluidics in the cultivation of cancer cell spheroids. Six patient-derived xenograft (PDX) tumors of high-grade serous ovarian cancer were used as the source material to dem

SUBMITTER: Dadgar N 

PROVIDER: S-EPMC8433468 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature