Double emulsion-pretreated microwell culture for the in vitro production of multicellular spheroids and their in situ analysis.

ABSTRACT: Multicellular spheroids have served as a promising preclinical model for drug efficacy testing and disease modeling. Many microfluidic technologies, including those based on water-oil-water double emulsions, have been introduced for the production of spheroids. However, sustained culture and the in situ characterization of the generated spheroids are currently unavailable for the double emulsion-based spheroid model. This study presents a streamlined workflow, termed the double emulsion-pretreated microwell culture (DEPMiC), incorporating the features of (1) effective initiation of uniform-sized multicellular spheroids by the pretreatment of double emulsions produced by microfluidics without the requirement of biomaterial scaffolds; (2) sustained maintenance and culture of the produced sph

SUBMITTER: Qu F 

PROVIDER: S-EPMC8433470 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature