MicroED structure of the human adenosine receptor determined from a single nanocrystal in LCP.

ABSTRACT: G protein-coupled receptors (GPCRs), or seven-transmembrane receptors, are a superfamily of membrane proteins that are critically important to physiological processes in the human body. Determining high-resolution structures of GPCRs without bound cognate signaling partners, such as a G protein, requires crystallization in lipidic cubic phase (LCP). GPCR crystals grown in LCP are often too small for traditional X-ray crystallography. These microcrystals are ideal for investigation by microcrystal electron diffraction (MicroED), but the gel-like nature of LCP makes traditional approaches to MicroED sample preparation insurmountable. Here, we show that the structure of a human A_2A adenosine receptor can be determined by MicroED after converting the LCP into the sponge phase follow

SUBMITTER: Martynowycz MW 

PROVIDER: S-EPMC8433539 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature