Amyloid particles facilitate surface-catalyzed cross-seeding by acting as promiscuous nanoparticles.

ABSTRACT: Amyloid seeds are nanometer-sized protein particles that accelerate amyloid assembly as well as propagate and transmit the amyloid protein conformation associated with a wide range of protein misfolding diseases. However, seeded amyloid growth through templated elongation at fibril ends cannot explain the full range of molecular behaviors observed during cross-seeded formation of amyloid by heterologous seeds. Here, we demonstrate that amyloid seeds can accelerate amyloid formation via a surface catalysis mechanism without propagating the specific amyloid conformation associated with the seeds. This type of seeding mechanism is demonstrated through quantitative characterization of the cross-seeded assembly reactions involving two nonhomologous and unrelated proteins: the human Aβ42 peptide

SUBMITTER: Koloteva-Levine N 

PROVIDER: S-EPMC8433567 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature