Immobilization of Phospholipase A1 Using a Protein-Inorganic Hybrid System.

ABSTRACT: In this study, four kinds of phospholipase A1-metal (Al/Co/Cu/Mn) hybrid nanostructures were prepared for enhancing the stability of the free PLA1. The formed hybrid complexes were characterized by scanning electron microscope (SEM), Fourier infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The stability and substrate specificity of immobilized enzymes were subsequently determined. After immobilization, the temperature tolerance of PLA1-metal hybrid nanostructures was enhanced. The relative activity of PLA1-Al/Co/Cu hybrid nanostructures remained above 60% at 50 °C, while that of free enzyme was below 5%. The thermal transition temperature measured by differential scanning calorimetry (DSC) was found to increase from 65.59 °C (free enzyme) to 173.14 °C, 123.67 °C, 96.31 °C, and 11

SUBMITTER: Cheng S 

PROVIDER: S-EPMC8433775 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature