AoiQ Catalyzes Geminal Dichlorination of 1,3-Diketone Natural Products.

ABSTRACT: Enzymes that can perform halogenation of aliphatic carbons are of significant interest to the synthetic and biocatalysis communities. Here we describe the characterization of AoiQ, a single-component flavin-dependent halogenase (FDH) that catalyzes gem-dichlorination of 1,3-diketone substrates in the biosynthesis of dichlorodiaporthin. AoiQ represents the first biochemically reconstituted FDH that can halogenate an enolizable sp³-hybridized carbon atom.

SUBMITTER: Liu M 

PROVIDER: S-EPMC8434754 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature