Extended-representation bisulfite sequencing of gene regulatory elements in multiplexed samples and single cells.

ABSTRACT: The biological roles of DNA methylation have been elucidated by profiling methods based on whole-genome or reduced-representation bisulfite sequencing, but these approaches do not efficiently survey the vast numbers of non-coding regulatory elements in mammalian genomes. Here we present an extended-representation bisulfite sequencing (XRBS) method for targeted profiling of DNA methylation. Our design strikes a balance between expanding coverage of regulatory elements and reproducibly enriching informative CpG dinucleotides in promoters, enhancers and CTCF binding sites. Barcoded DNA fragments are pooled before bisulfite conversion, allowing multiplex processing and technical consistency in low-input samples. Application of XRBS to single leukemia cells enabled us to evaluate genetic copy n

SUBMITTER: Shareef SJ 

PROVIDER: S-EPMC8434949 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature