Estradiol-driven metabolism in transwomen associates with reduced circulating extracellular vesicle microRNA-224/452.

ABSTRACT:

Objective

Sex steroid hormones like estrogens have a key role in the regulation of energy homeostasis and metabolism. In transwomen, gender-affirming hormone therapy like estradiol (in combination with antiandrogenic compounds) could affect metabolism as well. Given that the underlying pathophysiological mechanisms are not fully understood, this study assessed circulating estradiol-driven microRNAs (miRs) in transwomen and their regulation of genes involved in metabolism in mice.

Methods

Following plasma miR-sequencing (seq) in a transwomen discovery (n = 20) and validation cohort (n = 30), we identified miR-224 and miR-452. Subsequent systemic silencing of these miRs in male C57Bl/6 J mice (n = 10) was followed by RNA-seq-based gene expression analysis of brown and white ad

SUBMITTER: Florijn BW 

PROVIDER: S-EPMC8436186 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature