Development of a Csy4-processed guide RNA delivery system with soybean-infecting virus ALSV for genome editing.

ABSTRACT:

Background

A key issue for implementation of CRISPR-Cas9 genome editing for plant trait improvement and gene function analysis is to efficiently deliver the components, including guide RNAs (gRNAs) and Cas9, into plants. Plant virus-based gRNA delivery strategy has proven to be an important tool for genome editing. However, its application in soybean which is an important crop has not been reported yet. ALSV (apple latent spherical virus) is highly infectious virus and could be explored for delivering elements for genome editing.

Results

To develop a ALSV-based gRNA delivery system, the Cas9-based Csy4-processed ALSV Carry (CCAC) system was developed. In this system, we engineered the soybean-infecting ALSV to carry and deliver gRNA(s). The endoribonuclease Csy4 effectively

SUBMITTER: Luo Y 

PROVIDER: S-EPMC8436479 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature