R-loops and regulatory changes in chronologically ageing fission yeast cells drive non-random patterns of genome rearrangements.

ABSTRACT: Aberrant repair of DNA double-strand breaks can recombine distant chromosomal breakpoints. Chromosomal rearrangements compromise genome function and are a hallmark of ageing. Rearrangements are challenging to detect in non-dividing cell populations, because they reflect individually rare, heterogeneous events. The genomic distribution of de novo rearrangements in non-dividing cells, and their dynamics during ageing, remain therefore poorly characterized. Studies of genomic instability during ageing have focussed on mitochondrial DNA, small genetic variants, or proliferating cells. To characterize genome rearrangements during cellular ageing in non-dividing cells, we interrogated a single diagnostic measure, DNA breakpoint junctions, using Schizosaccharomyces pombe as a model system. Aberra

SUBMITTER: Ellis DA 

PROVIDER: S-EPMC8437301 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature