The β₂Tubulin, Rad50-ATPase and enolase cis-regulatory regions mediate male germline expression in Tribolium castaneum.

ABSTRACT: Genetics-based pest management processes, including the sterile insect technique, are an effective method for the control of some pest insects. However, current SIT methods are not directly transferable to many important pest insect species due to the lack of genetic sexing strains. Genome editing is revolutionizing the way we conduct genetics in insects, including in Tribolium castaneum, an important genetic model and agricultural pest. We identified orthologues of β₂Tubulin, Rad50-ATPase and enolase in T. castaneum. Using RT-PCR, we confirmed that these genes are predominantly expressed in the testis. PiggyBac-based transformation of T. castaneum cis-regulatory regions derived from Tc-β₂t, Tc-rad50 or Tc-eno resulted in EGFP expression specifically in the T. castane

SUBMITTER: Khan SA 

PROVIDER: S-EPMC8438054 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature