Spatioregional assessment of the gut microbiota in experimental necrotizing pancreatitis.

ABSTRACT: Infectious complications following experimental pancreatitis involve major disruptions in the gut microbiota. The aim of this study was to characterize this disruption by examining the spatioregional distribution in microbial community structure and function following experimental pancreatitis associated with pancreatic infection. Mice were subjected to infusion of the pancreatic duct with either taurocholate to induce necrotizing pancreatitis or normal saline (control group). The spatial (lumen versus mucosa) and regional composition and function of the microbiota from the duodenum, ileum, caecum, colon, pancreas and blood were evaluated using 16S rRNA gene amplicon sequencing. Mice that developed necrotizing pancreatitis demonstrated a decrease in microbial richness and significantly alt

SUBMITTER: van den Berg FF 

PROVIDER: S-EPMC8438261 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature