Functional analysis of peripheral and intratumoral neoantigen-specific TCRs identified in a patient with melanoma.

ABSTRACT:

Background

Neoantigens derived from somatic mutations correlate with therapeutic responses mediated by treatment with immune checkpoint inhibitors. Neoantigens are therefore highly attractive targets for the development of therapeutic approaches in personalized medicine, although many aspects of their quality and associated immune responses are not yet well understood. In a case study of metastatic malignant melanoma, we aimed to perform an in-depth characterization of neoantigens and respective T-cell responses in the context of immune checkpoint modulation.

Methods

Three neoantigens, which we identified either by immunopeptidomics or in silico prediction, were investigated using binding affinity analyses and structural simulations. We isolated seven T-cell receptors (TCRs)

SUBMITTER: Braunlein E 

PROVIDER: S-EPMC8438848 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature