Dual RNA-seq transcriptome analysis of caecal tissue during primary Eimeria tenella infection in chickens.

ABSTRACT:

Background

Coccidiosis is an infectious disease with large negative impact on the poultry industry worldwide. It is an enteric infection caused by unicellular Apicomplexan parasites of the genus Eimeria. The present study aimed to gain more knowledge about interactions between parasites and the host immune system during the early asexual replication phase of E. tenella in chicken caeca. For this purpose, chickens were experimentally infected with E. tenella oocysts, sacrificed on days 1-4 and 10 after infection and mRNA from caecal tissues was extracted and sequenced.

Results

Dual RNA-seq analysis revealed time-dependent changes in both host and parasite gene expression during the course of the infection. Chicken immune activation was detected from day 3 and onwards with the

SUBMITTER: Sandholt AKS 

PROVIDER: S-EPMC8438895 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature