Cancer derived exosomes induce macrophages immunosuppressive polarization to promote bladder cancer progression.

ABSTRACT:

Background

Exosomes mediated crosstalk between tumor cells and other stromal cells including tumor associated macrophages plays an essential role in reprogramming tumor microenvironment (TME) to facilitate tumor progression. However, the mechanism of tumor derived exosomes promotes bladder cancer progression have not been defined.

Methods

Exosomes were extracted from bladder cancer cells MB49 conditioned medium by ultracentrifugation. The effects of MB49-derived exosomes on macrophages polarization were analyzed by qPCR, flow cytometry, and Western blot. The immunosuppressive phenotype and function of MB49-derived exosomes stimulated macrophages were verified by tumor xenograft assays and T cell co-culture experiments. Exosomal miRNAs were analyzed by microarray to identify

SUBMITTER: Jiang Z 

PROVIDER: S-EPMC8439012 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature