Comprehensive analysis of lysine crotonylation modification in patients with chronic renal failure.

ABSTRACT:

Background

Post-translational modifications (PTMs) are at the heart of many cellular signaling events, which changes the function of protein. Crotonylation, one of the most important and common PTMs, plays a crucial role in the regulation of various biological processes. However, no study has evaluated the role of lysine crotonylation modification in chronic renal failure (CRF) patients.

Methods

Here, we comparatively evaluated the crotonylation proteome of normal controls and chronic renal failure patients using liquid chromatography-tandem mass spectrometry (LC-MS/MS) coupled with highly sensitive immune-affinity purification.

Results

A total of 1109 lysine modification sites were identified, of which 772 sites were up-regulated and 69 sites were down-regulated. Thi

SUBMITTER: Huang J 

PROVIDER: S-EPMC8439085 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature