Shape of promoter antisense RNAs regulates ligand-induced transcription activation.

ABSTRACT: The size of the transcriptional program of long non-coding RNAs in the mammalian genome has engendered discussions about their biological roles¹, particularly the promoter antisense (PAS) transcripts^2,3. Here we report the development of an assay-referred to as chromatin isolation by RNA-Cas13a complex-to quantitatively detect the distribution of RNA in the genome. The assay revealed that PAS RNAs serve as a key gatekeeper of a broad transcriptional pause release program, based on decommissioning the 7SK small nuclear RNA-dependent inhibitory P-TEFb complex. Induction of PAS RNAs by liganded ERα led to a significant loss of H3K9me3 and the release of basally recruited HP1α and KAP1 on activated target gene promoters. This release was due to PAS RNA-dependent recruitme

SUBMITTER: Yang F 

PROVIDER: S-EPMC8439151 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature