Dataset Information

Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.

ABSTRACT:

Context

Salidroside, a compound extracted from Rhodiola rosea L. (Crassulaceae), possesses many beneficial pathological effects.

Objective

To explore the effect of salidroside on ventilator-induced lung endothelial dysfunction in vivo and in vitro.

Materials and methods

In vivo, male ICR mice were divided into sham, ventilation, salidroside, and ventilation plus salidroside groups. The mice were ventilated for 4 h, salidroside (50 mg/kg) was administrated intraperitoneally before ventilation, dexamethasone (Dex) (5 mg/kg) was used as a positive control. In vitro, mouse lung vascular endothelial cells (MLVECs) were treated with salidroside, MMP-9 siRNA, and BAY11-7082 (10 μM), and then exposed to cyclic stretch for 4 h. Afterward, lung tissues and MLVECs were collected for further analysis.

Results

Salidroside pre-treatment significantly reversed the expression of vascular endothelial cadherin (VE-cadherin) and zonula occluden-1 (ZO-1) proteins in cyclic stretch-treated MLVECs (0.46 ± 0.09 vs. 0.80 ± 0.14, 0.49 ± 0.05 vs. 0.88 ± 0.08) and ventilated lung tissues (0.56 ± 0.06 vs. 0.83 ± 0.46, 0.49 ± 0.08 vs. 0.80 ± 0.12). The results further indicated that salidroside inhibited the expression of matrix metalloproteinase-9 (MMP-9), whereas knockdown of its expression restored the expression levels of VE-cadherin (0.37 ± 0.08 vs. 0.85 ± 0.74) and ZO-1 (0.48 ± 0.08 vs. 0.81 ± 0.11) in stretched MLVECs. Meanwhile, salidroside inhibited the NF-κB signalling pathway and alleviated lung injury.

Conclusions

Salidroside protected against stretch-induced endothelial barrier function, improving lung injury after ventilation. Thus, salidroside may be a promising therapeutic agent for patients with MV-induced lung injury.

SUBMITTER: Zhang H

PROVIDER: S-EPMC8439245 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Publications

Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.

Zhang Hui H Dong Wenwen W Li Siyuan S Zhang Yunqian Y Lv Zhou Z Yang Lu L Jiang Lai L Wu Tao T Wang Yan Y

Pharmaceutical biology 20211201 1

<h4>Context</h4>Salidroside, a compound extracted from <i>Rhodiola rosea</i> L. (Crassulaceae), possesses many beneficial pathological effects.<h4>Objective</h4>To explore the effect of salidroside on ventilator-induced lung endothelial dysfunction <i>in vivo</i> and <i>in vitro</i>.<h4>Materials and methods</h4><i>In vivo</i>, male ICR mice were divided into sham, ventilation, salidroside, and ventilation plus salidroside groups. The mice were ventilated for 4 h, salidroside (50 mg/kg) was admi ...[more]

PMID: 34517742

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Dataset Information

Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.

Context

Objective

Materials and methods

Results

Conclusions

Publications

Salidroside protects against ventilation-induced lung injury by inhibiting the expression of matrix metalloproteinase-9.

Similar Datasets

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