Assessment of serological assays for identifying high titer convalescent plasma.

ABSTRACT:

Background

The COVID-19 pandemic has been accompanied by the largest mobilization of therapeutic convalescent plasma (CCP) in over a century. Initial identification of high titer units was based on dose-response data using the Ortho VITROS IgG assay. The proliferation of severe acute respiratory syndrome coronavirus 2 serological assays and non-uniform application has led to uncertainty about their interrelationships. The purpose of this study was to establish correlations and analogous cutoffs between multiple serological assays.

Methods

We compared the Ortho, Abbott, Roche, an anti-spike (S) ELISA, and a virus neutralization assay. Relationships relative to FDA-approved cutoffs under the CCP emergency use authorization were identified in convalescent plasma from a cohort o

SUBMITTER: Farnsworth CW 

PROVIDER: S-EPMC8440373 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature