Biomimetic Biphasic Electrospun Scaffold for Anterior Cruciate Ligament Tissue Engineering.

ABSTRACT:

Background

Replacing damaged anterior cruciate ligaments (ACLs) with tissue-engineered artificial ligaments is challenging because ligament scaffolds must have a multiregional structure that can guide stem cell differentiation. Here, we designed a biphasic scaffold and evaluated its effect on human marrow mesenchymal stem cells (MSCs) under dynamic culture conditions as well as rat ACL reconstruction model in vivo.

Methods

We designed a novel dual-phase electrospinning strategy wherein the scaffolds comprised randomly arranged phases at the two ends and an aligned phase in the middle. The morphological, mechanical properties and scaffold degradation were investigated. MSCs proliferation, adhesion, morphology and fibroblast markers were evaluated under dynamic culturing. This

SUBMITTER: Tang Y 

PROVIDER: S-EPMC8440765 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature