Developmental Angiogenesis Requires the Mitochondrial Phenylalanyl-tRNA Synthetase.

ABSTRACT: Background: Mitochondrial aminoacyl-tRNA synthetases (mtARSs) catalyze the binding of specific amino acids to their cognate tRNAs and play an essential role in the synthesis of proteins encoded by mitochondrial DNA. Defects in mtARSs have been linked to human diseases, but their tissue-specific pathophysiology remains elusive. Here we examined the role of mitochondrial phenylalanyl-tRNA synthetase (FARS2) in developmental angiogenesis and its potential contribution to the pathogenesis of cardiovascular disease. Methods: Morpholinos were injected into fertilized zebrafish ova to establish an in vivo fars2 knock-down model. A visualization of the vasculature was achieved by using Tg (fli1: EGFP) ^y1 transgenic zebrafish. In addition, small interfe

SUBMITTER: Li B 

PROVIDER: S-EPMC8440837 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature