Desensitisation of Notch signalling through dynamic adaptation in the nucleus.

ABSTRACT: During embryonic development, signalling pathways orchestrate organogenesis by controlling tissue-specific gene expression programmes and differentiation. Although the molecular components of many common developmental signalling systems are known, our current understanding of how signalling inputs are translated into gene expression outputs in real-time is limited. Here we employ optogenetics to control the activation of Notch signalling during Drosophila embryogenesis with minute accuracy and follow target gene expression by quantitative live imaging. Light-induced nuclear translocation of the Notch Intracellular Domain (NICD) causes a rapid activation of target mRNA expression. However, target gene transcription gradually decays over time despite continuous photo-activation and nuclear N

SUBMITTER: Viswanathan R 

PROVIDER: S-EPMC8441390 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature