Disrupted macrophage metabolic reprogramming in aged soleus muscle during early recovery following disuse atrophy.

ABSTRACT: Aged skeletal muscle is characterized by poor muscle recovery following disuse coinciding with an impaired muscle pro-inflammatory macrophage response. Macrophage inflammatory status is regulated by its metabolic state, but little is understood of macrophage metabolism and its relation to macrophage inflammation in the context of muscle recovery and aging. Therefore, the purpose of this study was to thoroughly characterize macrophage metabolism and inflammation in aged muscle during early recovery following disuse atrophy using single cell transcriptomics and functional assays. Young (4-5 months) and old (20-22 months) male C57BL/6 mice underwent 14 days of hindlimb unloading followed by 4 days of ambulatory recovery. CD45+ cells were isolated from solei muscles and analyzed using 10x Geno

SUBMITTER: Fix DK 

PROVIDER: S-EPMC8441489 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature