SARS-CoV-2 infects an upper airway model derived from induced pluripotent stem cells.

ABSTRACT: As one of the primary points of entry of xenobiotic substances and infectious agents into the body, the lungs are subject to a range of dysfunctions and diseases that together account for a significant number of patient deaths. In view of this, there is an outstanding need for in vitro systems in which to assess the impact of both infectious agents and xenobiotic substances of the lungs. To address this issue, we have developed a protocol to generate airway epithelial basal-like cells from induced pluripotent stem cells, which simplifies the manufacture of cellular models of the human upper airways. Basal-like cells generated in this study were cultured on transwell inserts to allow formation of a confluent monolayer and then exposed to an air-liquid interface to induce differentiation int

SUBMITTER: Djidrovski I 

PROVIDER: S-EPMC8441770 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature