Dataset Information

Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development.

ABSTRACT: We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration and long-duration spaceflight. Previous studies have identified miRNAs that regulate rodent responses to spaceflight in low-Earth orbit, and we have confirmed the expression of these proposed spaceflight-associated miRNAs in rodents reacting to simulated spaceflight conditions. Moreover, astronaut samples from the NASA Twins Study confirmed these expression signatures in miRNA sequencing, single-cell RNA sequencing (scRNA-seq), and single-cell assay for transposase accessible chromatin (scATAC-seq) data. Additionally, a subset of these miRNAs (miR-125, miR-16, and let-7a) was found to regulate vascular damage caused by simulated deep space radiation. To demonstrate the physiological relevance of key spaceflight-associated miRNAs, we utilized antagomirs to inhibit their expression and successfully rescue simulated deep-space-radiation-mediated damage in human 3D vascular constructs.

SUBMITTER: Malkani S

PROVIDER: S-EPMC8441986 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature

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Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development.

Malkani Sherina S Chin Christopher R CR Cekanaviciute Egle E Mortreux Marie M Okinula Hazeem H Tarbier Marcel M Schreurs Ann-Sofie AS Shirazi-Fard Yasaman Y Tahimic Candice G T CGT Rodriguez Deyra N DN Sexton Brittany S BS Butler Daniel D Verma Akanksha A Bezdan Daniela D Durmaz Ceyda C MacKay Matthew M Melnick Ari A Meydan Cem C Li Sheng S Garrett-Bakelman Francine F Fromm Bastian B Afshinnekoo Ebrahim E Langhorst Brad W BW Dimalanta Eileen T ET Cheng-Campbell Margareth M Blaber Elizabeth E Schisler Jonathan C JC Vanderburg Charles C Friedländer Marc R MR McDonald J Tyson JT Costes Sylvain V SV Rutkove Seward S Grabham Peter P Mason Christopher E CE Beheshti Afshin A

Cell reports 20201125 10

We have identified and validated a spaceflight-associated microRNA (miRNA) signature that is shared by rodents and humans in response to simulated, short-duration and long-duration spaceflight. Previous studies have identified miRNAs that regulate rodent responses to spaceflight in low-Earth orbit, and we have confirmed the expression of these proposed spaceflight-associated miRNAs in rodents reacting to simulated spaceflight conditions. Moreover, astronaut samples from the NASA Twins Study conf ...[more]

PMID: 33242410

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Dataset Information

Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development.

Publications

Circulating miRNA Spaceflight Signature Reveals Targets for Countermeasure Development.

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