The detection of long-lasting memory foot-and-mouth disease (FMD) virus serotype O-specific CD4⁺ T cells from FMD-vaccinated cattle by bovine major histocompatibility complex class II tetramer.

ABSTRACT: Foot-and-mouth disease (FMD) is a highly contagious, economically devastating disease of cloven-hooved animals. The development of long-lasting effective FMD vaccines would greatly benefit the global FMD control programme. Deep analysis of adaptive immunity in cattle vaccinated against FMD is technically challenging due to the lack of species-specific tools. In this study, we aimed to identify CD4⁺ T-cell epitopes in the FMD virus (FMDV) capsid and to phenotype the CD4⁺ T cells that recognize them using bovine major histocompatibility complex (BoLA) class II tetramer. A BoLA class II tetramer based on the DRA/DRB3*020:02 allele and FMDV antigen-stimulated PBMCs from bovine vaccinates were used to successfully identify four epitopes in the FMDV capsid, three of which h

SUBMITTER: Mitoma S 

PROVIDER: S-EPMC8442236 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature