EIF5B regulates the expression of PD-L1 in prostate cancer cells by interacting with Wig1.

ABSTRACT:

Background

Eukaryotic translation initiation factors (eIFs) are the key factors to synthesize translation initiation complexes during the synthesis of eukaryotic proteins. Besides, eIFs are especially important in regulating the immune function of tumor cells. However, the effect mechanism of eIFs in prostate cancer remains to be studied, which is precisely the purpose of this study.

Methods

In this study, three groups of prostate cancer cells were investigated. One group had its eIF5B gene knocked down; another group had its Programmed death 1 (PD-L1) overexpressed; the final group had its Wild-type p53-induced gene 1 (Wig1) overexpressed. Genetic alterations of the cancer cells were performed by plasmid transfection. The expression of PD-L1 mRNA was detected by quantitativ

SUBMITTER: Li Q 

PROVIDER: S-EPMC8442339 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature