The 3D nuclear conformation of the major histocompatibility complex changes upon cell activation both in porcine and human macrophages.

ABSTRACT:

Background

The crucial role of the major histocompatibility complex (MHC) for the immune response to infectious diseases is well-known, but no information is available on the 3D nuclear organization of this gene-dense region in immune cells, whereas nuclear architecture is known to play an essential role on genome function regulation. We analyzed the spatial arrangement of the three MHC regions (class I, III and II) in macrophages using 3D-FISH. Since this complex presents major differences in humans and pigs with, notably, the presence of the centromere between class III and class II regions in pigs, the analysis was implemented in both species to determine the impact of this organization on the 3D conformation of the MHC. The expression level of the three genes selected to repres

SUBMITTER: Mompart F 

PROVIDER: S-EPMC8442435 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature