Toward Comprehensive Plasma Proteomics by Orthogonal Protease Digestion.

ABSTRACT: Rapid and consistent protein identification across large clinical cohorts is an important goal for clinical proteomics. With the development of data-independent technologies (DIA/SWATH-MS), it is now possible to analyze hundreds of samples with great reproducibility and quantitative accuracy. However, this technology benefits from empirically derived spectral libraries that define the detectable set of peptides and proteins. Here, we apply a simple and accessible tip-based workflow for the generation of spectral libraries to provide a comprehensive overview on the plasma proteome in individuals with and without active tuberculosis (TB). To boost protein coverage, we utilized nonconventional proteases such as GluC and AspN together with the gold standard trypsin, identifying more than 30,00

SUBMITTER: Fossati A 

PROVIDER: S-EPMC8442619 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature