RE-SELEX: restriction enzyme-based evolution of structure-switching aptamer biosensors.

ABSTRACT: Aptamers are widely employed as recognition elements in small molecule biosensors due to their ability to recognize small molecule targets with high affinity and selectivity. Structure-switching aptamers are particularly promising for biosensing applications because target-induced conformational change can be directly linked to a functional output. However, traditional evolution methods do not select for the significant conformational change needed to create structure-switching biosensors. Modified selection methods have been described to select for structure-switching architectures, but these remain limited by the need for immobilization. Herein we describe the first homogenous, structure-switching aptamer selection that directly reports on biosensor capacity for the target. We exploit th

SUBMITTER: Sanford AA 

PROVIDER: S-EPMC8442683 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature