Valorization of lignin components into gallate by integrated biological hydroxylation, O-demethylation, and aryl side-chain oxidation.
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ABSTRACT:
SUBMITTER: Cai C
PROVIDER: S-EPMC8442903 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
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Valorization of lignin components into gallate by integrated biological hydroxylation, O-demethylation, and aryl side-chain oxidation.
Cai Chenggu C Xu Zhaoxian Z Zhou Huarong H Chen Sitong S Jin Mingjie M
Science advances 20210901 36
Converting lignin components into a single product is a promising way to upgrade lignin. Here, an efficient biocatalyst was developed to selectively produce gallate from lignin components by integrating three main reactions: hydroxylation, O-demethylation, and aryl side-chain oxidation. A rationally designed hydroxylase system was first introduced into a gallate biodegradation pathway–blocked <i>Rhodococcus opacus</i> mutant so that gallate accumulated from protocatechuate and compounds in its u ...[more]
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