Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish.

ABSTRACT: Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes to both muscle growth and regeneration. Upon exposure to activating signals, MuSCs proliferate to generate myoblasts that differentiate and fuse to grow or regenerate myofibres. This myogenic progression resembles aspects of muscle formation and development during embryogenesis. Therefore, the study of MuSCs and their associated myofibres permits the exploration of muscle stem cell biology, including the cellular and molecular mechanisms underlying muscle formation, maintenance and repair. As most aspects of MuSC biology have been described in

SUBMITTER: Ganassi M 

PROVIDER: S-EPMC8443456 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature