Master regulator NtrC controls the utilization of alternative nitrogen sources in Pseudomonas stutzeri A1501.

ABSTRACT: Pseudomonas stutzeri A1501 is a model strain used to study associative nitrogen fixation, and it possesses the nitrogen regulatory NtrC protein in the core genome. Nitrogen sources represent one of the important factors affecting the efficiency of biological nitrogen fixation in the natural environment. However, the regulation of NtrC during nitrogen metabolism in P. stutzeri A1501 has not been clarified. In this work, a phenotypic analysis of the ntrC mutant characterized the roles of NtrC in nitrogen metabolism and the oxidative stress response of P. stutzeri A1501. To systematically identify NtrC-controlled gene expression, RNA-seq was performed to further analyse the gene expression differences between the wild-type strain and the ∆ntrC mutant under nitrogen fixation conditions. A tota

SUBMITTER: Yang Z 

PROVIDER: S-EPMC8443478 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature