Effects of Semen Processing on Sperm Function: Differences between Swim-Up and Density Gradient Centrifugation.

ABSTRACT:

Purpose

Andrology research has evolved notoriously in the latest years, particularly since male factor contribution to couple infertility has been undoubtedly demonstrated. However, sperm function investigations results are sometimes contradictory, probably as a result of the use of different sperm processing techniques. In this work, we underwent a systematic functional comparison of human sperm samples simultaneously processed by swim-up and density gradient centrifugation, which are the preferred sperm processing methods used in basic and clinical laboratories.

Materials and methods

To compare functional characteristics of sperm isolated by swim-up and density gradient centrifugation followed by incubation at different times under capacitating conditions.

Results

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SUBMITTER: Hernandez-Silva G 

PROVIDER: S-EPMC8443982 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature