DNA methylation and gene expression profiles to identify childhood atopic asthma associated genes.

ABSTRACT:

Background

Asthma is a chronic inflammatory disorder of the airways involving many different factors. This study aimed to screen for the critical genes using DNA methylation/CpGs and miRNAs involved in childhood atopic asthma.

Methods

DNA methylation and gene expression data (Access Numbers GSE40732 and GSE40576) were downloaded from the Gene Expression Omnibus database. Each set contains 194 peripheral blood mononuclear cell (PBMC) samples of 97 children with atopic asthma and 97 control children. Differentially expressed genes (DEGs) with DNA methylation changes were identified. Pearson correlation analysis was used to select genes with an opposite direction of expression and differences in methylation levels, and then Gene Ontology (GO) function and Kyoto Encyclopedia of

SUBMITTER: Chen R 

PROVIDER: S-EPMC8444351 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature