MicroRNA-550a-3-5p controls the brain metastasis of lung cancer by directly targeting YAP1.

ABSTRACT:

Background

This study aimed to explore the potential regulatory mechanisms of brain metastasis and to identify novel underlying targets of lung cancer with brain metastasis.

Methods

Exosomes were isolated from the plasma of lung cancer patients with or without brain metastasis and low or high metastatic lung cancer cells, and small RNA from plasma-derived exosomes were sequenced. Differentially expressed miRNAs (DE-miRNAs) were identified. Human brain microvascular endothelial cells (HBMECs) were transfected with miR-550a-3-5p mimics or inhibitors and exosomes. Cell viability, migration, and apoptosis/cycle were determined using Cell Counting Kit-8 (CCK-8), Transwell, and flow cytometry, respectively. Western blotting was used to measure the expression of the associated prot

SUBMITTER: Wei L 

PROVIDER: S-EPMC8444378 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature