Effect of epigallocatechin gallate on dental biofilm of Streptococcus mutans: An in vitro study.

ABSTRACT:

Background

Streptococcus mutans (S. mutans) plays a major role in the formation of dental caries. The aim of this study was to examine the effect of the green tea polyphenol, epigallocatechin gallate (EGCG), on biofilm formation of S. mutans.

Methods

Following exposure to increasing concentrations of EGCG, the planktonic growth was measured by optical density and the biofilm biomass was quantified by crystal violet staining. Exopolysaccharides (EPS) production was visualized by confocal scanning laser microscopy, and the bacterial DNA content was determined by quantitative polymerase chain reaction (qPCR). Gene expression of selected genes was analyzed by real time (RT)-qPCR and membrane potential was examined by flow cytometry.

Results

We observed that EGCG inhibited

SUBMITTER: Schneider-Rayman M 

PROVIDER: S-EPMC8444437 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature