Sustained release and protein stabilization reduce the growth factor dosage required for human pluripotent stem cell expansion.

ABSTRACT: Translation of human pluripotent stem cell (hPSC)-derived therapies to the clinic demands scalable, cost-effective methods for cell expansion. Culture media currently used for hPSC expansion rely on high concentrations and frequent supplementation of recombinant growth factors due to their short half-life at physiological temperatures. Here, we developed a biomaterial strategy using mineral-coated microparticles (MCMs) to sustain delivery of basic fibroblast growth factor (bFGF), a thermolabile protein critical for hPSC pluripotency and proliferation. We show that the MCMs stabilize bFGF against thermally induced activity loss and provide more efficient sustained release of active growth factor compared to polymeric carriers commonly used for growth factor delivery. Using a statistically d

SUBMITTER: Khalil AS 

PROVIDER: S-EPMC8445021 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature