Pitching single-focus confocal data analysis one photon at a time with Bayesian nonparametrics.

ABSTRACT: Fluorescence time traces are used to report on dynamical properties of molecules. The basic unit of information in these traces is the arrival time of individual photons, which carry instantaneous information from the molecule, from which they are emitted, to the detector on timescales as fast as microseconds. Thus, it is theoretically possible to monitor molecular dynamics at such timescales from traces containing only a sufficient number of photon arrivals. In practice, however, traces are stochastic and in order to deduce dynamical information through traditional means-such as fluorescence correlation spectroscopy (FCS) and related techniques-they are collected and temporally autocorrelated over several minutes. So far, it has been impossible to analyze dynamical properties of molecules

SUBMITTER: Tavakoli M 

PROVIDER: S-EPMC8445401 | biostudies-literature | 2020 Jan-Mar

REPOSITORIES: biostudies-literature