Reassembling protein complexes after controlled disassembly by top-down mass spectrometry in native mode.

ABSTRACT: The combined use of electrospray ionization run in so-called "native mode" with top-down mass spectrometry (nTDMS) is enhancing both structural biology and discovery proteomics by providing three levels of information in a single experiment: the intact mass of a protein or complex, the masses of its subunits and non-covalent cofactors, and fragment ion masses from direct dissociation of subunits that capture the primary sequence and combinations of diverse post-translational modifications (PTMs). While intact mass data are readily deconvoluted using well-known software options, the analysis of fragmentation data that result from a tandem MS experiment - essential for proteoform characterization - is not yet standardized. In this tutorial, we offer a decision-tree for the analysis of nTDMS

SUBMITTER: Schachner LF 

PROVIDER: S-EPMC8445521 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature