Visualizing endogenous Rho activity with an improved localization-based, genetically encoded biosensor.

ABSTRACT: Rho GTPases are regulatory proteins, which orchestrate cell features such as morphology, polarity and movement. Therefore, probing Rho GTPase activity is key to understanding processes such as development and cell migration. Localization-based reporters for active Rho GTPases are attractive probes to study Rho GTPase-mediated processes in real time with subcellular resolution in living cells and tissue. Until now, relocation Rho biosensors (sensors that relocalize to the native location of active Rho GTPase) seem to have been only useful in certain organisms and have not been characterized well. In this paper, we systematically examined the contribution of the fluorescent protein and Rho-binding peptides on the performance of localization-based sensors. To test the performance, we compared

SUBMITTER: Mahlandt EK 

PROVIDER: S-EPMC8445605 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature