Citrate Synthase and OGDH as Potential Biomarkers of Atherosclerosis under Chronic Stress.

ABSTRACT:

Background

Pathological changes of the adrenal gland and the possible underlying molecular mechanisms are currently unclear in the case of atherosclerosis (AS) combined with chronic stress (CS).

Methods

New Zealand white rabbits were used to construct a CS and AS animal model. Proteomics and bioinformatics were employed to identify hub proteins in the adrenal gland related to CS and AS. Hub proteins were detected using immunohistochemistry, immunofluorescence assays, and Western blotting. Real-time quantitative polymerase chain reaction (RT-qPCR) was used to analyze the expression of genes. In addition, a neural network model was constructed. The quantitative relationships were inferred by cubic spline interpolation. Enzymatic activity of mitochondrial citrate synthase and O

SUBMITTER: Meng LB 

PROVIDER: S-EPMC8445721 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature