A novel antioxidant ergothioneine PET radioligand for in vivo imaging applications.

ABSTRACT: Ergothioneine (ERGO) is a rare amino acid mostly found in fungi, including mushrooms, with recognized antioxidant activity to protect tissues from damage by reactive oxygen species (ROS) components. Prior to this publication, the biodistribution of ERGO has been performed solely in vitro using extracted tissues. The aim of this study was to develop a feasible chemistry for the synthesis of an ERGO PET radioligand, [¹¹C]ERGO, to facilitate in vivo study. The radioligand probe was synthesized with identical structure to ERGO by employing an orthogonal protection/deprotection approach. [¹¹C]methylation of the precursor was performed via [¹¹C]CH₃OTf to provide [¹¹C]ERGO radioligand. The [¹¹C]ERGO was isolated by RP-HPLC with a m

SUBMITTER: Behof WJ 

PROVIDER: S-EPMC8446031 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature