Dataset Information

Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species.

ABSTRACT: Poly(ethylene terephthalate) (PET) is a petroleum-based plastic that is massively produced and used worldwide. A promising PET recycling process to circumvent petroleum feedstock consumption and help to reduce environmental pollution is microbial or enzymatic biodegradation of post-consumer (PC) PET packages to its monomers-terephthalic acid (TPA) and ethylene glycol (EG)-or to key intermediates in PET synthesis-such as mono- and bis-(2-hydroxyethyl) terephthalate (MHET and BHET). Two species of filamentous fungi previously characterized as lipase producers (Penicillium restrictum and P. simplicissimum) were evaluated in submerged fermentation for induction of lipase production by two inducers (BHET and amorphous PET), and for biodegradation of two substrates (BHET and PC-PET). BHET induced lipase production in P. simplicissimum, achieving a peak of 606.4 U/L at 49 h (12.38 U/L.h), representing an almost twofold increase in comparison to the highest peak in the control (without inducers). Microbial biodegradation by P. simplicissimum after 28 days led to a 3.09% mass loss on PC-PET fragments. In contrast, enzymatic PC-PET depolymerization by cell-free filtrates from a P. simplicissimum culture resulted in low concentrations of BHET, MHET and TPA (up to 9.51 µmol/L), suggesting that there are mechanisms at the organism level that enhance biodegradation. Enzymatic BHET hydrolysis revealed that P. simplicissimum extracellular enzymes catalyze the release of MHET as the predominant product. Our results show that P. simplicissimum is a promising biodegrader of PC-PET that can be further explored for monomer recovery in the context of feedstock recycling processes.

SUBMITTER: Moyses DN

PROVIDER: S-EPMC8446152 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using <i>Penicillium</i> species.

Moyses Danuza N DN Teixeira Danielle A DA Waldow Vinicius A VA Freire Denise M G DMG Castro Aline M AM

3 Biotech 20210916 10

Poly(ethylene terephthalate) (PET) is a petroleum-based plastic that is massively produced and used worldwide. A promising PET recycling process to circumvent petroleum feedstock consumption and help to reduce environmental pollution is microbial or enzymatic biodegradation of post-consumer (PC) PET packages to its monomers-terephthalic acid (TPA) and ethylene glycol (EG)-or to key intermediates in PET synthesis-such as mono- and bis-(2-hydroxyethyl) terephthalate (MHET and BHET). Two species of ...[more]

PMID: 34603913

Similar Datasets

Project description:Glycolysis of post-consumer polyethylene terephthalate (PET) waste is a promising chemical recycling technique, back to the monomer, bis(2-hydroxyethyl) terephthalate (BHET). This work presents sodium methoxide (MeONa) as a low-cost catalyst for this purpose. BHET product was confirmed by gas chromatography-mass spectrometry (GCMS), Nuclear Magnetic Resonance (NMR) Spectroscopy, melting point, and Differential Scanning Calorimetry (DSC). It was shown, not surprisingly, that PET conversion increases with the glycolysis temperature. At a fixed temperature of 190 °C, the response surface methodology (RSM) based on the Box-Behnken design was applied. Four independent factors, namely the molar ratio of PET: MeONa (50-150), the molar ratio of ethylene glycol to PET (EG: PET) (3-7), the reaction time (2-6 h), and the particle size (0.25-1 mm) were studied. Based on the experimental results, regression models as a function of significant process factors were obtained and evaluated by analysis of variance (ANOVA), to predict the depolymerization performance of MeONa in terms of PET conversion. Coefficient of determination, R2 of 95% indicated the adequacy for predicted model. Afterward, the regression model was validated and optimized within the design space with a prediction of 87% PET conversion at the optimum conditions demonstrating a deviation of less than 5% from predicted response. A van 't Hoff plot confirmed the endothermic nature of the depolymerization reaction. The ceiling temperature (TC = 160 °C) was calculated from Gibbs' free energy. A kinetic study for the depolymerization reaction was performed and the activation energy for MeONa was estimated from the Arrhenius plot (EA = 130 kJ/mol). The catalytic depolymerization efficiency of MeONa was compared under similar conditions with widely studied zinc acetate and cobalt acetate. This study shows that MeONa's performance, as a glycolysis catalyst is promising; in addition, it is much cheaper and environmentally more benign than heavy metal salts. These findings make a valuable contribution towards the chemical recycling of post-consumer PET waste to meet future recycling demands of a circular economy.

Dataset Information

Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using Penicillium species.

Publications

Fungal and enzymatic bio-depolymerization of waste post-consumer poly(ethylene terephthalate) (PET) bottles using <i>Penicillium</i> species.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets