Minimal residual disease assessment in acute lymphoblastic leukemia by 4-color flow cytometry: Recommendations from the MRD Working Group of the Brazilian Society of Bone Marrow Transplantation.

ABSTRACT:

Introduction

The minimal residual disease (MRD) status plays a crucial role in the treatment of acute lymphoblastic leukemia (ALL) and is currently used in most therapeutic protocols to guide the appropriate therapeutic decision. Therefore, it is imperative that laboratories offer accurate and reliable results through well standardized technical processes by establishing rigorous operating procedures.

Method

Our goal is to propose a monoclonal antibody (MoAb) panel for MRD detection in ALL and provide recommendations intended for flow cytometry laboratories that work on 4-color flow cytometry platforms.

Results and conclusion

The document includes pre-analytical and analytical procedures, quality control assurance, technical procedures, as well as the information that

SUBMITTER: Ikoma-Colturato MRV 

PROVIDER: S-EPMC8446261 | biostudies-literature | 2021 Jul-Sep

REPOSITORIES: biostudies-literature