Regulation of the Muscarinic M₃ Receptor by Myocardin-Related Transcription Factors.

ABSTRACT: Myocardin-related transcription factors (MRTFs: myocardin/MYOCD, MRTF-A/MRTFA, and MRTF-B/MRTFB) are co-factors of serum response factor (SRF) that activate the smooth muscle cell (SMC) gene program and that play roles in cardiovascular development and mechanobiology. Gain and loss of function experiments have defined the SMC gene program under control of MRTFs, yet full understanding of their impact is lacking. In the present study, we tested the hypothesis that the muscarinic M₃ receptor (CHRM3) is regulated by MRTFs together with SRF. Forced expression of MYOCD (8d) in human coronary artery (SMC) followed by RNA-sequencing showed increased levels of M₂, M₃, and M₅ receptors (CHRM2: 2-fold, CHRM3: 16-fo

SUBMITTER: Liu L 

PROVIDER: S-EPMC8446542 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature