Identification of covalent modifications regulating immune signaling complex composition and phenotype.

ABSTRACT: Cells signal through rearrangements of protein communities governed by covalent modifications and reversible interactions of distinct sets of proteins. A method that identifies those post-transcriptional modifications regulating signaling complex composition and functional phenotypes in one experimental setup would facilitate an efficient identification of novel molecular signaling checkpoints. Here, we devised modifications, interactions and phenotypes by affinity purification mass spectrometry (MIP-APMS), comprising the streamlined cloning and transduction of tagged proteins into functionalized reporter cells as well as affinity chromatography, followed by MS-based quantification. We report the time-resolved interplay of more than 50 previously undescribed modification and hundreds of pr

SUBMITTER: Frauenstein A 

PROVIDER: S-EPMC8447602 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature